April 10, 2019

Emily Anderson Coauthored a Paper on Denitrifying Bacteria in Woodchip Bioreactors for Frontiers in Microbiology

Emily Anderson (Minnesota) coauthored a paper entitled "Denitrifying Bacteria Active in Woodchip Bioreactors at Low-Temperature Conditions" published in the journal Frontiers in Microbiology starting on page 635 in volume 10 on April 2, 2019.

Her coauthors were Jeonghwan Jang, Rodney T. Venterea, Michael J. Sadowsky, Carl J. Rosen, Gary W. Feyereisen, and Satoshi Ishii.

Emily is a Staff Scientist based in Minnesota focused on bioremediation, environmental microbiology, and bioinformatics/data analysis. A remediation and water treatment practitioner, she is a key contributer to projects in industries including mining, agribusiness, manufacturing and refining. Her prior experience includes work at New Zealand Petroleum and Minerals and the Ministry of Social Development.

Frontiers in Microbiology is a leading journal in its field, publishing peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an Editorial Board of international researchers. This multidisciplinary open-access journal disseminates and communicates scientific knowledge and discoveries to researchers, academics, clinicians and the public worldwide.

Abstract

Woodchip bioreactor technology removes nitrate from agricultural subsurface drainage by using denitrifying microorganisms. Although woodchip bioreactors have demonstrated success in many field locations, low water temperature can significantly limit bioreactor efficiency and performance. To improve bioreactor performance, it is important to identify the microbes responsible for nitrate removal at low temperature conditions. Therefore, in this study, we identified and characterized denitrifiers active at low-temperature conditions by using culture-independent and -dependent approaches. By comparative 16S rRNA (gene) analysis and culture isolation technique, Pseudomonas spp., Polaromonas spp., and Cellulomonas spp. were identified as being important bacteria responsible for denitrification in woodchip bioreactor microcosms at relatively low temperature conditions (15∘C). Genome analysis of Cellulomonas sp. strain WB94 confirmed the presence of nitrite reductase gene nirK. Transcription levels of this nirK were significantly higher in the denitrifying microcosms than in the non-denitrifying microcosms. Strain WB94 was also capable of degrading cellulose and other complex polysaccharides. Taken together, our results suggest that Cellulomonas sp. denitrifiers could degrade woodchips to provide carbon source and electron donors to themselves and other denitrifiers in woodchip bioreactors at low-temperature conditions. By inoculating these denitrifiers (i.e., bioaugmentation), it might be possible to increase the nitrate removal rate of woodchip bioreactors at low-temperature conditions.

More Information

Read the article: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00635/full
Learn more about the journal: https://www.frontiersin.org/journals/microbiology#about
For consultation regarding denitrifying bacteria, contact Emily Anderson at This email address is being protected from spambots. You need JavaScript enabled to view it..
Learn more about Emily at: https://www.linkedin.com/in/emily-anderson-59303085/

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