Bioaugmentation to Treat Chlorinated Solvents in Fractured Bedrock

Geosyntec's use of KB-1 at this former manufacturing facility has enabled us to reduce TCE concentrations in groundwater by over 98%. Geosyntec's use of KB-1 at this former manufacturing facility has enabled us to reduce TCE concentrations in groundwater by over 98%.

Historic releases of chlorinated solvents at this former electronics manufacturing site led to the presence of elevated concentrations of chlorinated solvents in the subsurface. Geosyntec was retained with the primary remedial goal being to reduce concentrations of chlorinated solvents in the intermediate bedrock zone, approximately 70 to 110 feet below ground surface. The overall objective was to achieve treatment of the contaminated groundwater while minimizing the impact on plans to redevelop the site.

Geosyntec's Scope of Services

Geosyntec determined that native microorganisms in the subsurface had the ability to degrade the target compounds, principally trichloroethene (TCE), but in some cases, this native dechlorinating activity was stalling with the production of daughter compounds such as vinyl chloride (VC) and cis-1,2-dichloroethene (cis-DCE). The relatively high concentrations of these compounds indicated that reductive dehalogenation was not resulting in complete dechlorination of TCE to simpler, non-toxic breakdown compounds.

Based on these conditions, bioaugmentation was selected as the remediation technology to be field tested. Emulsified soybean oil with lactate (electron donor) and KB-1, a natural, nonpathogenic microbial consortium containing several strains of Dehalococcoides ethenogenes, were chosen for testing. Two methods of culture and donor addition were performed; passive injection in one area of the site, and injection followed by temporary groundwater pumping (to induce a hydraulic gradient and draw the injected materials through the rock fractures) in another.

Preliminary data and field observations indicated that the passive injection was unable to achieve electron donor and KB-1 delivery to the two observation wells 12 and 24 feet away. The injection with the induced hydraulic gradient, however, was able to distribute the electron donor at least 42 feet from the injection well (to the second observation well). Additionally, microbial analysis one month after injection indicated that the KB-1 culture was present in the extraction well.

After the first two months, data showed that TCE concentrations in groundwater in the vicinity of the passive injection well decreased from over 800 micrograms per liter to less than 100 micrograms per liter. The induced hydraulic gradient injection had similar results, with TCE concentrations dropping from over 120 micrograms per liter to less than 2 micrograms per liter in the injection well, and similar results in the observation wells 30 and 42 feet away. In addition, cis-DCE and VC concentrations were being reduced as the contaminants were completely degraded to ethene and other innocuous compounds. Two years post-injection, there has been no increase in the TCE concentrations, indicating that the combination of the KB-1 culture and a long term donor may be preventing rebound from matrix diffusion.

Notable Accomplishments

In just a matter of months, Geosyntec's use of Dehalococcoides ethenogenes to bioremediate chlorinated solvents in fractured bedrock has resulted in initial remediation rates of over 98%. In addition, due to the relatively small size of the system required to accomplish this, site redevelopment activities have been able to progress unimpeded, saving significant time and expense for the site owner.

Project Overview

  • Location: Bloomfield, New Jersey, USA
  • Client: Confidential
  • Project Practice Areas: Contaminated Sites
  • Type of Facility: Maunufacturing Site
  • Services Provided: Pilot-Test Design; Technical Support; Field Implementation of Bioaugmentation; Performance Monitoring; Conceptual Full Scale Design
  • Type of Work: Bioaugmentation
  • Company: Geosyntec Consultants
  • Governing Regulation: State Regulations
Geosyntec Consultants
Geosyntec is a consulting and engineering firm that works with private and public sector clients to address new ventures and complex problems involving our environment, natural resources, and civil infrastructure.

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